[Effect of quercetin on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells]
[Effect of quercetin on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells]
OBJECTIVE: To investigate the effect of quercetin (Que) on HEN1 cells, a kind of human nasopharyngeal carcinoma cells. METHOD: Inhibition rate of quercetin on HEN1 was assayed by MTT method, cell apoptosis by flow cytometry (FCM), Caspase-3 expression of each group were determined by colorimetry set. RESULT: The inhibition of quercetin on HEN1 cells was shown in the dose-dependent (r = 0.709, P < 0.05) and time-dependent manner (r = 0.703, P < 0.01). The ratio of apoptotic and necrosis cells increased accompanied with added quercetin concentration. Cell cycle was specificly arrested inG2/M phase. Apoptosis cusp was revealed by FCM. While the activity of caspase-3 were also significantly upregulated in five groups after quecetin compared to control (P < 0.05). CONCLUSION: Quecetin can activate the expression of Caspase-3 and induce the apoptosis of HEN1 cells through mitochondrion-depended pathway.
Zhang F, Cui Y, Cao P.
Department of Otorhinolaryngology-Head and Neck Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. lhkzhangfeng@163.com